| Primary Identifier | MGI:3849051 | Allele Type | Targeted |
| Attribute String | Inducible, Null/knockout, Recombinase | Gene | Lmx1b |
| Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| description | Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. |
| molecularNote | A C->A transition (changing the asparagine N, at position 246 to lysine, K) was introduced into exon 5 to produce a null allele of Lmx1b. Subsequently, an IREScreERT2 cassette, followed by a loxP-flanked PGK-neo, was inserted into the 3'UTR of the locus by homologous recombination in PC3 ES cells. Cre mediated recombination then removed the PGK-neo cassette upon transmission from male chimeric mice. The protamine-cre transgene contributed by the PC3 cells was separated from the knock-in allele by crossing germline male mutants with C57BL/6 mice. |
