| First Author | Fuhrmann F | Year | 2016 |
| Journal | Elife | Volume | 5 |
| PubMed ID | 28035902 | Mgi Jnum | J:239541 |
| Mgi Id | MGI:5829134 | Doi | 10.7554/eLife.20616 |
| Citation | Fuhrmann F, et al. (2016) Adequate immune response ensured by binary IL-2 and graded CD25 expression in a murine transfer model. Elife 5:e20616 |
| abstractText | The IL-2/IL-2Ralpha (CD25) axis is of central importance for the interplay of effector and regulatory T cells. Nevertheless, the question how different antigen loads are translated into appropriate IL-2 production to ensure adequate responses against pathogens remains largely unexplored. Here we find that at single cell level, IL-2 is binary (digital) and CD25 is graded expressed whereas at population level both parameters show graded expression correlating with the antigen amount. Combining in vivo data with a mathematical model we demonstrate that only this binary IL-2 expression ensures a wide linear antigen response range for Teff and Treg cells under real spatiotemporal conditions. Furthermore, at low antigen concentrations binary IL-2 expression safeguards by its spatial distribution selective STAT5 activation only of closely adjacent Treg cells regardless of their antigen specificity. These data show that the mode of IL-2 secretion is critical to tailor the adaptive immune response to the antigen amount. |
