| Primary Identifier | MGI:3850723 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Igh |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neomycin resistance gene, a splice accepter, an internal ribosome entry site, a SV40 large and small T antigen coding region, and an extra copy of the IgH intronic enhancer Emu were inserted in a reverse orientation between DQ52 and JH1 in the IgH locus. The neomycin gene was removed after targeting by crossing founder mice with transgenic mice expressing cre in the germline. SV40 T antigen transcripts were detected by RT-PCR in B cells. |
