| Primary Identifier | MGI:3851472 | Allele Type | Targeted |
| Gene | Shh | Transmission | Germline |
| Strain of Origin | 129S4/SvJae | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Mutations (arginine codon 34 (AGG) to alanine (GCG) (p.R34A) and lysine codon 38 (AAA) to alanine (GCA) (p.K38A)) were introduced into the N-terminal Cardin-Weintraub motif encode by exon 1 to reduce high-affinity Shh-proteoglycan interaction without altering Shh's affinity for its receptor. For targeting purposes, a floxed neomycin cassette was introduced into intron 1 and subsequently removed by transient expression of cre recombinase. Expression levels of the mutant protein in homozygotes was identical to wild-type as determined by immunoblot analysis of cerebellar extracts. |
