| Primary Identifier | MGI:3966900 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Rfx2 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A lacZ reporter poly A gene cassette with a splice acceptor was inserted into intron 1 of the gene before the ATG start codon in exon 2. Transcription at the locus should result in the splicing of exon 1 to the gene trap vector and eliminate the production of the endogenous transcript. RT-PCR analysis of total RNA from E9.5 embryos confirmed the absence of detectable mRNA in homozygous mutant samples. X-gal staining revealed that lacZ activity was present in ciliated tissues during mouse development including the node, the floor plate and the dorsal neural tube. |
