| First Author | Mildner A | Year | 2017 |
| Journal | Eur J Immunol | Volume | 47 |
| Issue | 7 | Pages | 1142-1152 |
| PubMed ID | 28471480 | Mgi Jnum | J:246250 |
| Mgi Id | MGI:5924414 | Doi | 10.1002/eji.201746987 |
| Citation | Mildner A, et al. (2017) MicroRNA-142 controls thymocyte proliferation. Eur J Immunol 47(7):1142-1152 |
| abstractText | T-cell development is a spatially and temporally regulated process, orchestrated by well-defined contributions of transcription factors and cytokines. Here, we identify the noncoding RNA miR-142 as an additional regulatory layer within murine thymocyte development and proliferation. MiR-142 deficiency impairs the expression of cell cycle-promoting genes in mature mouse thymocytes and early progenitors, accompanied with increased levels of cyclin-dependent kinase inhibitor 1B (Cdkn1b, also known as p27Kip1 ). By using CRISPR/Cas9 technology to delete the miR-142-3p recognition element in the 3'UTR of cdkn1b, we confirm that this gene is a novel target of miR-142-3p in vivo. Increased Cdkn1b protein expression alone however was insufficient to cause proliferation defects in thymocytes, indicating the existence of additional critical miR-142 targets. Collectively, we establish a key role for miR-142 in the control of early and mature thymocyte proliferation, demonstrating the multifaceted role of a single miRNA on several target genes. |
