Primary Identifier | MGI:6316964 | Allele Type | Targeted |
Attribute String | Recombinase | Gene | Slc6a3 |
Transmission | Germline | Strain of Origin | C57BL/6NCrl |
Is Recombinase | true | Is Wild Type | false |
molecularNote | The DAT-ires-Flpo targeting construct was designed to insert a loxN-flanked neomycin resistance (neo) cassette followed by an internal ribosomal entry site (IRES) and a Flpo recombinase sequence downstream of the stop codon of the solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3) gene. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to CMV-cre mice to remove the neo cassette. |