| First Author | García R | Year | 2016 |
| Journal | Cell Signal | Volume | 28 |
| Issue | 10 | Pages | 1563-79 |
| PubMed ID | 27418101 | Mgi Jnum | J:329561 |
| Mgi Id | MGI:6851409 | Doi | 10.1016/j.cellsig.2016.07.003 |
| Citation | Garcia R, et al. (2016) Extracellular heat shock protein 90 binding to TGFbeta receptor I participates in TGFbeta-mediated collagen production in myocardial fibroblasts. Cell Signal 28(10):1563-79 |
| abstractText | The pathological remodeling heart shows an increase in left ventricular mass and an excess of extracellular matrix deposition that can over time cause heart failure. Transforming growth factor beta (TGFbeta) is the main cytokine controlling this process. The molecular chaperone heat shock protein 90 (Hsp90) has been shown to play a critical role in TGFbeta signaling by stabilizing the TGFbeta signaling cascade. We detected extracellular Hsp90 in complex with TGFbeta receptor I (TGFbetaRI) in fibroblasts and determined a close proximity between both proteins suggesting a potential physical interaction between the two at the plasma membrane. This was supported by in silico studies predicting Hsp90 dimers and TGFbetaRI extracellular domain interaction. Both, Hsp90aa1 and Hsp90ab1 isoforms participate in TGFbetaRI complex. Extracellular Hsp90 inhibition lessened the yield of collagen production as well as the canonical TGFbeta signaling cascade, and collagen protein synthesis was drastically reduced in Hsp90aa1 KO mice. These observations together with the significant increase in activity of Hsp90 at the plasma membrane pointed to a functional cooperative partnership between Hsp90 and TGFbetaRI in the fibrotic process. We propose that a surface population of Hsp90 extracellularly binds TGFbetaRI and this complex behaves as an active participant in collagen production in TGFbeta-activated fibroblasts. We also offer an in vivo insight into the role of Hsp90 and its isoforms during cardiac remodeling in murine aortic banding model suffering from pathological cardiac remodeling and detect circulating Hsp90 overexpressed in remodeling mice. |
