| Primary Identifier | MGI:4128052 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Sec23a |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A gene trap vector inserted into intron 2. The resulting fusion transcript of the first two exons of the gene (encoding the first 73 amino acids) with the beta-geo cassette produces a chimeric protein missing over 90% of the coding region, which is expected to be nonfunctional. Western blot analysis using an antibody recognizing an internal epitope near the C-terminus confirmed the absence of protein expression in homozygous mutant embryonic fibroblast cell lysates. |
