| First Author | Mehrotra P | Year | 2015 |
| Journal | Immunology | Volume | 146 |
| Issue | 4 | Pages | 537-46 |
| PubMed ID | 26222149 | Mgi Jnum | J:246258 |
| Mgi Id | MGI:5924611 | Doi | 10.1111/imm.12515 |
| Citation | Mehrotra P, et al. (2015) Poly-ADP-ribosyl polymerase-14 promotes T helper 17 and follicular T helper development. Immunology 146(4):537-46 |
| abstractText | Transcription factors are critical determinants of T helper cell fate and require a variety of co-factors to activate gene expression. We previously identified the ADP ribosyl-transferase poly-ADP-ribosyl polymerase 14 (PARP-14) as a co-factor of signal transducer and activator of transcription (STAT) 6 that is important in B-cell and T-cell responses to interleukin-4, particularly in the differentiation of T helper type 2 (Th2) cells. However, whether PARP-14 functions during the development of other T helper subsets is not known. In this report we demonstrate that PARP-14 is highly expressed in Th17 cells, and that PARP-14 deficiency and pharmacological blockade of PARP activity result in diminished Th17 differentiation in vitro and in a model of allergic airway inflammation. We further show that PARP-14 is expressed in T follicular helper (Tfh) cells and Tfh cell development is impaired in PARP-14-deficient mice following immunization with sheep red blood cells or inactivated influenza virus. Decreases in Th17 and Tfh development are correlated with diminished phospho-STAT3 and decreased expression of the interleukin-6 receptor alpha-chain in T cells. Together, these studies demonstrate that PARP-14 regulates multiple cytokine responses during inflammatory immunity. |
