| Primary Identifier | MGI:4330764 | Allele Type | Gene trapped |
| Attribute String | Hypomorph, Reporter | Gene | Ccnt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene trap vector inserted into intron 3 of the gene. The insertion of the trap sequence introduces a splice acceptor, a stop codon and a strong polyadenylation site, thus splicing nascent transcripts of the Ccnt1 gene to the trap to create a fusion protein between CCNT1 and beta-geo, which contains only 18% of CCNT1 amino acid sequence. Western blot analysis of cell lysates from various organs demonstrated that, when normalized to levels of total protein, homozygous mutant mice exhibit some residual (less than 5%) expression of CCNT1 protein relative to wild-type controls. |
