| Primary Identifier | MGI:4340648 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Stk38l |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Trapped gene association determined by genome coordinate overlap and strand. The position of the gene trap was identified with a 5' rapid amplification of cDNA ends (5'RACE) sequence tag matching exons 9-7 of the gene and thus indicating the gene trap location in intron 9. Serial PCR and sequence analyses showed that the gene trap vector integrated at position bp 995 of intron 9 of the gene. Western blot analysis confirmed absence of full-length protein in the hippocampus and the presence of a Stk38l(1-282)::beta-geo fusion protein that has most of the activating sequence, the kinase subdomains VIII-XII, including the substrate recognition sites and the c-terminal hydrophobic motif deleted. |
