Primary Identifier | MGI:4353628 | Allele Type | Targeted |
Gene | Gt(ROSA)26Sor | Transmission | Germline |
Strain of Origin | (C57BL/6J x 129S6/SvEvTac)F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Using RMCE, an inserted hygro cassette was replaced with a neo cassette and a short hairpin RNA driven by the human U6 promoter that targets Crhr1. Expression of the shRNA results in an 80% reduction in endogenous Crhr1 transcript levels compared to in wild-type mice. |