| Primary Identifier | MGI:4356069 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Rfk |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A frt-flanked neomycin cassette and an upstream loxP site was inserted upstream of exon 1. A second loxP site was inserted downstream of exon 2 at nucleotide position 1080. Founder mice were generated from homologously targeted clones and then crossed with Flp-deleter to remove the neomycin cassette and leave exons 1 and 2 floxed. Correct targeting was confirmed by Southern blotting. |
