| Primary Identifier | MGI:4355877 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Cd1d1 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An EYFP expression cassette and a floxed neomycin resistance cassette were inserted in-frame downstream of the last coding exon but upstream of the 3' untranslated region. Founder mice were crossed with cre-deleter mice to remove the neomycin cassette, leaving behind a loxP site. Immunoblot analysis of homozygote thymocyte extracts detected expression of just the fusion product. The fusion product was also detectable by flow cytometry. Developmental defects in a subset of homozygote NK T cells suggest some function of the endogenous protein is lost. |
