Mice containing the floxed Ccl2 allele were crossed with EIIa-Cre mice to obtain mice lacking exons 1 and 2. Correct targeting was confirmed by Southern blotting. Supernatant analysis of activated homozygote macrophages did not detect protein product confirming this is a null allele. Similar ELISA analysis demonstrated an increase in MCP-3 secretion, which is encoded by the nearby Ccl7 gene.
