| First Author | Virostko J | Year | 2010 |
| Journal | Mol Imaging Biol | Volume | 12 |
| Issue | 1 | Pages | 42-53 |
| PubMed ID | 19548035 | Mgi Jnum | J:152066 |
| Mgi Id | MGI:4355882 | Doi | 10.1007/s11307-009-0240-1 |
| Citation | Virostko J, et al. (2010) Bioluminescence Imaging in Mouse Models Quantifies beta Cell Mass in the Pancreas and After Islet Transplantation. Mol Imaging Biol 2(1):42-53 |
| abstractText | PURPOSE: We developed a mouse model that enables non-invasive assessment of changes in beta cell mass. PROCEDURES: We generated a transgenic mouse expressing luciferase under control of the mouse insulin I promoter [mouse insulin promoter-luciferase-Vanderbilt University (MIP-Luc-VU)] and characterized this model in mice with increased or decreased beta cell mass and after islet transplantation. RESULTS: Streptozotocin-induced, diabetic MIP-Luc-VU mice had a progressive decline in bioluminescence that correlated with a decrease in beta cell mass. MIP-Luc-VU animals fed a high-fat diet displayed a progressive increase in bioluminescence that reflected an increase in beta cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and could be imaged for more than a year. CONCLUSIONS: Bioluminescence in the MIP-Luc-VU mouse model is proportional to beta cell mass in the setting of increased and decreased beta cell mass and after transplantation. |
