| Primary Identifier | MGI:4359650 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Themis |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A construct containing GFP and a floxed neomycin cassette were used to replace exon 1 of the gene locus. The neomycin cassette was removed after targeting. GFP expression was detected in the double-positive and single-positive thymocytes as determined by flow cytometry. Endogenous protein was not expressed in homozygote thymocytes. |
