| Primary Identifier | MGI:4359729 | Allele Type | Targeted |
| Gene | Dp(7Herc2-Mkrn3)1Taku | Transmission | Germline |
| Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Sequential rounds of targeting inserted the selection cassettes and loxP sites proximal to Herc2 and distal to Mkrn3. A double-targeted clone in which the targeting had occurred on the homologous chromosomes (trans) was transiently transfected with a Cre recombinase in ES cells to induce recombination between the loxP sites on opposite chromosomes, which generated clones with a balanced duplication and deletion of 6.3 Mb region between Herc2 and Mkrn3. Only duplicated allele can be recovered after meiosis and mating. |
