| Primary Identifier | MGI:4361392 | Allele Type | Targeted |
| Gene | Gabra1 | Transmission | Germline |
| Strain of Origin | 129X1/SvJ | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The targeting vector was designed (by site-directed mutagenesis) to substitute a serine to histidine at amino acid position 270 and leucine to alanine at amino acid position 277 of exon 9. An FRT-flanked neomycin cassette was inserted upstream of exon 9. Chimeric animals were crossed to C57BL/6J and the F1 progeny crossed to B6.Cg-Tg(ACTFLPe)9205Dym/J (actin-FLPe) to remove the neo cassette and leave a single FRT site upstream of exon 9. |
