| Primary Identifier | MGI:4362302 | Allele Type | Targeted |
| Attribute String | Conditional ready, Hypomorph, Null/knockout, Reporter | Gene | Iftap |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Special note about mutant cell line EPD0067_1_B06 used in J:236765: The mutation generated in this ES cell line results in a hypomorphic allele. RT-PCR analysis revealed that synthesis of full-length transcript is strongly suppressed but not completely abrogated in homozygous mutant testes. |
| molecularNote | The L1L2_gt0 cassette was inserted at position 101413619 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 101414384. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
