| Primary Identifier | MGI:4362969 | Allele Type | Targeted |
| Attribute String | Conditional ready, Hypomorph, Null/knockout, Reporter | Gene | Ulk4 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Special note about mutant cell line EPD0182_4_E12: The mutation generated in this ES cell line results in a hypomorphic allele. qRT-PCR analysis revealed a 78-96% reduction of mRNA expression in homozygous mutant mice (J:234631). |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 121088838 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 121089573. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
