| First Author | Ryder E | Year | 2014 |
| Journal | Transgenic Res | Volume | 23 |
| Issue | 1 | Pages | 177-85 |
| PubMed ID | 24197666 | Mgi Jnum | J:213544 |
| Mgi Id | MGI:5585251 | Doi | 10.1007/s11248-013-9764-x |
| Citation | Ryder E, et al. (2014) Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase. Transgenic Res 23(1):177-85 |
| abstractText | We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits. |
