| Primary Identifier | MGI:4364219 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Sel1l |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Conflicting reports exist for the integrity of the ES mutant cell line EPD0284_3_D08. A published report (J:206568) describes the line as carrying the Sel1ltm1a(KOMP)Wtsi allele and the Sel1ltm1c(KOMP)Wtsi allele derived from it. However, analysis at the Wellcome Trust Sanger Institute suggests that this ES cell line only carries the Sel1ltm1e(KOMP)Wtsi allele. |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 91797973 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
