Primary Identifier | MGI:4365636 | Allele Type | Transgenic |
Attribute String | Inserted expressed sequence | Gene | Tg(Vim*R113C)1Tmm |
Strain of Origin | CBA | Is Recombinase | false |
Is Wild Type | false |
molecularNote | An 18 kb segment containing 10.5 kb of 5'- and 1 kb of 3'-regulatory sequence was isolated from a BALB/c cosmid library. The myc epitope was introduced by site-directed mutagenesis after serine at the second amino acid position.An additional mutation was introduced by site-directed mutagenesis that resulted in an arginine to cytosine change at amino acid residue 113. The transgenic mouse line was generated from a founder animal expressing the mutant at sub-stoichiometric level (<=30%) compared with the endogenous gene. |