Primary Identifier | MGI:4367446 | Allele Type | Transgenic |
Attribute String | Recombinase | Gene | Tg(CAG-dre)1Afst |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A plasmid in which the CAG (CAGGS) promoter drives the mammalian codon-improved version of the bacteriophage D6 site-specific recombinase (dreo). This is followed by an IRES-puromycin sequence for selection. The linearized construct was electroporated into R1 ES cells, and single-copy clones were injected into 8-cell embryos. Germline transmission was obtained for one of these clones. |