| First Author | Zhang ZH | Year | 2020 |
| Journal | J Biol Chem | PubMed ID | 33262216 |
| Mgi Jnum | J:304932 | Mgi Id | MGI:6690346 |
| Doi | 10.1074/jbc.RA120.016485 | Citation | Zhang ZH, et al. (2020) Proteasome subunit alpha4s is essential for formation of spermatoproteasomes and histone degradation during meiotic DNA repair in spermatocytes. J Biol Chem |
| abstractText | Meiosis, which produces haploid progeny, is critical to ensuring both faithful genome transmission and genetic diversity. Proteasomes play critical roles at various stages of spermatogenesis, including meiosis, but the underlying mechanisms remain unclear. The atypical proteasomes, which contain the activator PA200, catalyze the acetylation-dependent degradation of the core histones in elongated spermatids and DNA repair in somatic cells. We show here that the testis-specific proteasome subunit alpha4s/PSMA8 is essential for male fertility by promoting proper formation of spermatoproteasomes, which harbor both PA200 and constitutive catalytic subunits. Immunostaining of a spermatocyte marker, SYCP3, indicated that meiosis was halted at stage of spermatocytes in the alpha4s-deficient testes. alpha4s stimulated the in vitro degradation of the acetylated core histones, instead of non-acetylated histones, by the PA200-proteasome. Deletion of alpha4s blocked degradation of the core histones at DNA damage loci in spermatocytes, leading to meiotic arrest at metaphase I. Thus, alpha4s is required for histone degradation at meiotic DNA damage loci, proper progression of meiosis, and fertility in males by promoting proper formation of spermatoproteasomes. These results are important for understanding male infertility, and might provide potential targets for male contraception or treatment of male infertility. |
