| Primary Identifier | MGI:4397704 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(Pgk1-EGFP)1Medv |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The transgenic construct has the Pgk1 promoter and the EGFP gene separated by a floxed STOP cassette containing a puromycin resistance selection marker, two poly A sites and a trasncriptional pause cassette (synthetic pA site and a transcriptional pause site). The linearized construct was electroporated into E14TG2a subclone IV ES cells. Positive clones were injected into day 3.5 C57BL/6 blastocysts and chimeras were bred to C57BL/6 mice to establish the transgenic line. |
