| Primary Identifier | MGI:4399711 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ldah |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-Regeneron |
| molecularNote | The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 18006bp between positions 8270605-8288610 of Chromosome 12 (Build GRCm39). Sequencing confirmed replacement of a region from exon 2, downstream of the start codon, to base 169 of exon 3, including the predicted catalytic Gx-SxG motif, with a lacZ reporter and a loxP-flanked neomycin cassette. Western blot analysis using an antibody binding a C-terminal antigen revealed no detectable protein in the liver, BAT and WAT, adrenal gland, prostate, brain, spleen, kidney, testis, and bone marrow-derived macrophages of homozygous mutant mice. Mass spectrometry analysis confirmed the absence of encoded peptides in WAT and liver lysates. |
