| Primary Identifier | MGI:4418949 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cttn |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A single loxP site was introduced 0.5-kb downstream of Exon 4. A reporter fusion construct containing a FRT site, splice acceptor (derived from 0.6kb upstream of exon 6), the cDNA from exons 6-18, IRES, b-galactosidase-neo, polyA, FRT site and an additional loxP site were inserted within intron 5 by homologous recombination. The reporter construct and exon 5 were removed by flp- and cre- mediated recombination respectively. Western blotting showed absence of protein in fibroblasts. |
