Primary Identifier | MGI:4419253 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Rec114 |
Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
Project Collection | KOMP-CSD |
description | Cell line EPD0392_4_D08 was successfully used to make chimeric mice. Germline transmission was accomplished. J:271216 |
molecularNote | The insertion of the L1L2_Bact_P cassette created a deletion of size 5762 starting at position 58567450 and ending at position 58573212 of Chromosome 9 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. |