| Primary Identifier | MGI:4419477 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Rhot2 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Cell line EPD0389_5_A05 was successfully used to make chimeric mice. Germline transmission was accomplished. J:240500 |
| molecularNote | The insertion of the L1L2_Bact_P cassette created a deletion of size 4102 starting at position 26059195 and ending at position 26063297 of Chromosome 17 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. |