| First Author | Xia Y | Year | 2010 |
| Journal | Genetics | Volume | 186 |
| Issue | 4 | Pages | 1139-46 |
| PubMed ID | 20923982 | Mgi Jnum | J:165696 |
| Mgi Id | MGI:4838106 | Doi | 10.1534/genetics.110.121160 |
| Citation | Xia Y, et al. (2010) Bulk segregation mapping of mutations in closely related strains of mice. Genetics 186(4):1139-46 |
| abstractText | Phenovariance may be obscured when genetic mapping is performed using highly divergent strains, and closely similar strains are preferred if adequate marker density can be established. We sequenced the C57BL/10J mouse genome using the Applied Biosystems SOLiD platform and here describe a genome-wide panel of informative markers that permits the mapping of mutations induced on the closely related C57BL/6J background by outcrossing to C57BL/10J, and backcrossing or intercrossing. The panel consists of 127 single nucleotide polymorphisms validated by capillary sequencing: 124 spaced at approximately 20-Mb intervals across the 19 autosomes, and three markers on the X chromosome. We determined the genetic relationship between four C57BL-derived substrains and used the panel to map two N-ethyl-N-nitrosourea (ENU)-induced mutations responsible for visible phenotypes in C57BL/6J mice through bulk segregation analysis. Capillary sequencing, with computation of relative chromatogram peak heights, was used to determine the proportion of alleles from each strain at each marker. |
