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Allele : Gt(ROSA)26Sor<tm1(CAG-EGFP)Fsh> gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Gordon Fishell
Primary Identifier  MGI:4420759 Allele Type  Targeted
Attribute String  Conditional ready, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  129S6/SvEvTac
Is Recombinase  false Is Wild Type  false
molecularNote  The R26R CAG-boosted EGFP (RCE) reporter targeting vector was designed with (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a splice acceptor site, a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA and 3x-polyA), an FRT-flanked modified pBS302 STOP cassette, an enhanced green fluorescent protein (EGFP) sequence, and two AttP-polyA sequences. After removal of the flanked STOP cassettes via cre- and Flp-mediated recombination, the EGFP reporter is expressed in cells/tissues where the expression patterns of the individual promoters driving Cre recombinase and FLP recombinase overlap.
  • mutations:
  • Insertion
  • synonyms:
  • RCE:dual,
  • RCE:dual
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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1 Carried By

0 Driven By

27 Publication categories





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