| First Author | Sakamoto M | Year | 2022 |
| Journal | Cell Rep Methods | Volume | 2 |
| Issue | 2 | Pages | 100168 |
| PubMed ID | 35474964 | Mgi Jnum | J:330745 |
| Mgi Id | MGI:7384595 | Doi | 10.1016/j.crmeth.2022.100168 |
| Citation | Sakamoto M, et al. (2022) A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Rep Methods 2(2):100168 |
| abstractText | Genetically encoded calcium indicators (GECIs) are widely used to measure calcium transients in neuronal somata and processes, and their use enables the determination of action potential temporal series in a large population of neurons. Here, we generate a transgenic mouse line expressing a highly sensitive green GECI, G-CaMP9a, in a Flp-dependent manner in excitatory and inhibitory neuronal subpopulations downstream of a strong CAG promoter. Combining this reporter mouse with viral or mouse genetic Flp delivery methods produces a robust and stable G-CaMP9a expression in defined neuronal populations without detectable detrimental effects. In vivo two-photon imaging reveals spontaneous and sensory-evoked calcium transients in excitatory and inhibitory ensembles with cellular resolution. Our results show that this reporter line allows long-term, cell-type-specific investigation of neuronal activity with enhanced resolution in defined populations and facilitates dissecting complex dynamics of neural networks in vivo. |
