| Primary Identifier | MGI:4421436 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Zfpm1 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| description | AA- p.Asn3Ala, p.Lys5Ala |
| molecularNote | A cDNA encoding two point mutations in the FOG repression motif (asparagine 3 to alanine and lysine 5 to alanine), which disrupt the interaction of the protein with subunits of the nucleosome remodeling and histone deacetylase complex, and a floxed neo cassette within intron 1, was inserted by homologous recombination. The neo cassette was removed by cre-mediated recombination. Western blot analysis in bone marrow cell lysates from homozygous mice confirmed expression of the mutant protein at levels similar to those of the wild-type protein. |
