| First Author | Hoang T | Year | 2022 |
| Journal | Cell Rep | Volume | 39 |
| Issue | 11 | Pages | 110849 |
| PubMed ID | 35705053 | Mgi Jnum | J:326141 |
| Mgi Id | MGI:7293996 | Doi | 10.1016/j.celrep.2022.110849 |
| Citation | Hoang T, et al. (2022) Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina. Cell Rep 39(11):110849 |
| abstractText | Direct reprogramming of glia into neurons is a potentially promising approach for the replacement of neurons lost to injury or neurodegenerative disorders. Knockdown of the polypyrimidine tract-binding protein Ptbp1 has been recently reported to induce efficient conversion of retinal Mupsilonller glia into functional neurons. Here, we use a combination of genetic lineage tracing, single-cell RNA sequencing (scRNA-seq), and electroretinogram analysis to show that selective induction of either heterozygous or homozygous loss-of-function mutants of Ptbp1 in adult retinal Mupsilonller glia does not lead to any detectable level of neuronal conversion. Only a few changes in gene expression are observed in Mupsilonller glia following Ptbp1 deletion, and glial identity is maintained. These findings highlight the importance of using genetic manipulation and lineage-tracing methods in studying cell-type conversion. |
