| First Author | Ma JH | Year | 2018 |
| Journal | Biochem Biophys Res Commun | Volume | 503 |
| Issue | 4 | Pages | 2646-2652 |
| PubMed ID | 30107916 | Mgi Jnum | J:270657 |
| Mgi Id | MGI:6277548 | Doi | 10.1016/j.bbrc.2018.08.017 |
| Citation | Ma JH, et al. (2018) CTCF is required for maintenance of auditory hair cells and hearing function in the mouse cochlea. Biochem Biophys Res Commun 503(4):2646-2652 |
| abstractText | Auditory hair cells play an essential role in hearing. These cells convert sound waves, mechanical stimuli, into electrical signals that are conveyed to the brain via spiral ganglion neurons. The hair cells are located in the organ of Corti within the cochlea. They assemble in a special arrangement with three rows of outer hair cells and one row of inner hair cells. The proper differentiation and preservation of auditory hair cells are essential for acquiring and maintaining hearing function, respectively. Many genetic regulatory mechanisms underlying hair-cell differentiation and maintenance have been elucidated to date. However, the role of epigenetic regulation in hair-cell differentiation and maintenance has not been definitively demonstrated. CTCF is an essential epigenetic component that plays a primary role in the organization of global chromatin architecture. To determine the role of CTCF in mammalian hair cells, we specifically deleted Ctcf in developing hair cells by crossing Ctcf(fl/fl) mice with Gfi1(Cre/+) mice. Gfi1(Cre); Ctcf(fl/fl) mice did not exhibit obvious developmental defects in hair cells until postnatal day 8. However, at 3 weeks, the Ctcf deficiency caused intermittent degeneration of the stereociliary bundles of outer hair cells, resulting in profound hearing impairment. At 5 weeks, most hair cells were degenerated in Gfi1(Cre); Ctcf(fl/fl) mice, and defects in other structures of the organ of Corti, such as the tunnel of Corti and Nuel's space, became apparent. These results suggest that CTCF plays an essential role in maintaining hair cells and hearing function in mammalian cochlea. |
