| Primary Identifier | MGI:4441057 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Pag1 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neomycin resistance cassette was inserted in intron 1 and a third loxP site was placed in intron 2 just downstream of exon 2. The selection cassette was then removed from properly targeted ES cells by transient cre expression. Homozygous mice were crossed to Tg(EIIa-cre)C5379Lmgd mice to delete exon 2. Western blot of protein from thymocytes and splenocytes of mice homozygous for the exon 2 deletion lacked expression of native protein and trucated forms. |
