| Primary Identifier | MGI:4443061 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Muc1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | Downstream of the translation termination codon of the endogenous gene was inserted an internal ribosomal entry site (IRES) followed by DNA encoding Cre recombinase joined to ERT2 and a frt site-flanked neomycin resistance cassette (IRES-CreERT2-FRT-neoR-FRT). ERT2 is a triply mutated (G400V, M543A, L544A) human estrogen receptor ligand-binding domain (LBD) that renders the fusion protein highly sensitive to activation by 4-hydroxytamoxifen, but unresponsive to the receptor's native ligands. |
