| Primary Identifier | MGI:4442798 | Allele Type | Targeted |
| Attribute String | Modified isoform(s), Null/knockout | Gene | Trp73 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 3' was replaced with a neo cassette by homologous recombination. This targets the delta Np73 isoforms but not TAp73 isoforms. The loss of Np73 isoform mRNA expression was confirmed by quantitative RT-PCR in MEFs and western blot analysis of lung tissue confirmed loss of isoform specific protein expression. Quantitative RT-PCR of liver, testis, and lung confirmed the retention of TAp73 isoform mRNA expression. |
