| Primary Identifier | MGI:4442611 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Fzd2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The coding region for a nuclear-localized beta-galactosidase (nLacZ; using an SV40 nuclear localization signal) was targeted to the Fzd1 locus by homologous recombination. The nLacZ sequences replace the Fzd2 open reading frame. A LoxP-flanked neo selectable marker used for ES cell selection was removed by subsequent crossing to a mouse line expressing germline Cre recombinase. Sequences from the E coli tetracycline resistance gene (TetR) are included immediately 3' of the nLacZ sequences and are part of the 3' untranslated region of the nLacZ transcript. |
