Primary Identifier | MGI:4442616 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Fzd7 |
Transmission | Germline | Strain of Origin | 129 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The open reading frame of the targeted gene was replaced by nuclear-localized beta-galactosidase (nLacZ; using an SV40 nuclear localization signal) using homologous recombination in a 129-derived embryonic stem (ES) cell line. A LoxP-flanked neomycin selectable marker used for ES cell selection was removed by subsequent crossing to a mouse line expressing germline Cre recombinase. |