| First Author | Luo C | Year | 2017 |
| Journal | Science | Volume | 357 |
| Issue | 6351 | Pages | 600-604 |
| PubMed ID | 28798132 | Mgi Jnum | J:243556 |
| Mgi Id | MGI:5909110 | Doi | 10.1126/science.aan3351 |
| Citation | Luo C, et al. (2017) Single-cell methylomes identify neuronal subtypes and regulatory elements in mammalian cortex. Science 357(6351):600-604 |
| abstractText | The mammalian brain contains diverse neuronal types, yet we lack single-cell epigenomic assays that are able to identify and characterize them. DNA methylation is a stable epigenetic mark that distinguishes cell types and marks regulatory elements. We generated >6000 methylomes from single neuronal nuclei and used them to identify 16 mouse and 21 human neuronal subpopulations in the frontal cortex. CG and non-CG methylation exhibited cell type-specific distributions, and we identified regulatory elements with differential methylation across neuron types. Methylation signatures identified a layer 6 excitatory neuron subtype and a unique human parvalbumin-expressing inhibitory neuron subtype. We observed stronger cross-species conservation of regulatory elements in inhibitory neurons than in excitatory neurons. Single-nucleus methylomes expand the atlas of brain cell types and identify regulatory elements that drive conserved brain cell diversity. |
