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Allele : Gt(ROSA)26Sor<tm6(Gli1)Amc> gene trap ROSA 26, Philippe Soriano; targeted mutation 6, Andrew P McMahon

Primary Identifier  MGI:4460761 Allele Type  Targeted
Attribute String  Conditional ready, Inserted expressed sequence, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  129 and C57BL/6 and CBA and Swiss Webster
Is Recombinase  false Is Wild Type  false
description  ES cell line = YFP3.1, a male ES cell line carrying Tg(CAG-cre/Esr1*)5Amc and is compound heterozgous at Gt(ROSA)26Sor: Gt(ROSA)26Sortm1Sor and Gt(ROSA)26Sortm1(EYFP)Cos.
molecularNote  A full-length Gli1 cDNA followed by sequence for a C-terminal 3X FLAG tag, an IRES, the Venus fluorescent reporter gene, an intron and poly(A) signal sequences were inserted into the locus downstream of a splice acceptor and loxP site flanked neomycin resistance gene cassette. Upon cre expression, the neo cassette is removed and FLAG tagged Gli1 gene and Venus gene expressed.
  • mutations:
  • Insertion
  • synonyms:
  • Rosa<Gli1-FLAG>,
  • R26-Gli1,
  • Rosa<Gli1-FLAG>,
  • R26-Gli1
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1 Feature

Genome

1 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

0 Driven By

11 Publication categories