| Primary Identifier | MGI:4818439 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Hoxb8 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Lox511 sites were inserted into the 5' and 3' UTRs of the Hoxb8 gene. An FRT-flanked neo selection cassette followed the 3' lox511 site. For temporal control of gene activation, an IRES-rtTA2M2 seqment was also introduced into the 3' UTR, along with an FRT-flanked neo selection cassette. The neo was subsequently removed by breeding with FLPe deleter animals. |
