| First Author | Shinkura R | Year | 2003 |
| Journal | Nat Immunol | Volume | 4 |
| Issue | 5 | Pages | 435-41 |
| PubMed ID | 12679811 | Mgi Jnum | J:97050 |
| Mgi Id | MGI:3574197 | Doi | 10.1038/ni918 |
| Citation | Shinkura R, et al. (2003) The influence of transcriptional orientation on endogenous switch region function. Nat Immunol 4(5):435-41 |
| abstractText | Immunoglobulin heavy chain (IgH) class switch recombination (CSR) takes place between large switch (S) regions that precede exons of the constant region. The precise functions of the S region are controversial, although transcription of the S region targets CSR. We have tested the effects of deletion, inversion and replacement of the endogenous 12-kilobase S(gamma1) region on CSR in vivo. Here we show that S(gamma1) is required for CSR, that CSR is effected by a 1-kilobase sequence that generates a G-rich transcript, and that inversion of S(gamma1) or the G-rich sequence decreases CSR. We conclude that S(gamma1) function is dependent on orientation and lacks an absolute requirement for common S region motifs. We propose that single-stranded DNA stabilized by transcription-dependent, higher order structures is a primary substrate of CSR. |
