| Primary Identifier | MGI:4822020 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tbx2 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A FRT flanked neo cassette with a 5' loxP site was inserted within intron 1 and an additional loxP site was inserted within intron 2 by homologous recombination. The selection cassette and exon 2 were removed by flpe and cre mediated recombination. Deletion of Exon 2 is expected to cause the loss of the domain essential for DNA binding and protein interactions and introduces a frame shift resulting in two stop codons in Exon 3 and early termination of translation. |
