| First Author | Badirou I | Year | 2014 |
| Journal | Blood | Volume | 124 |
| Issue | 16 | Pages | 2564-8 |
| PubMed ID | 25185263 | Mgi Jnum | J:218698 |
| Mgi Id | MGI:5618208 | Doi | 10.1182/blood-2014-06-584995 |
| Citation | Badirou I, et al. (2014) Carboxyl-terminal-dependent recruitment of nonmuscle myosin II to megakaryocyte contractile ring during polyploidization. Blood 124(16):2564-8 |
| abstractText | Endomitosis is a unique megakaryocyte (MK) differentiation process that is the consequence of a late cytokinesis failure associated with a contractile ring defect. Evidence from in vitro studies has revealed the distinct roles of 2 nonmuscle myosin IIs (NMIIs) on MK endomitosis: only NMII-B (MYH10), but not NMII-A (MYH9), is localized in the MK contractile ring and implicated in mitosis/endomitosis transition. Here, we studied 2 transgenic mouse models in which nonmuscle myosin heavy chain (NMHC) II-A was genetically replaced either by II-B or by a chimeric NMHCII that combined the head domain of II-A with the rod and tail domains of II-B. This study provides in vivo evidence on the specific role of NMII-B on MK polyploidization. It demonstrates that the carboxyl-terminal domain of the heavy chains determines myosin II localization to the MK contractile ring and is responsible for the specific role of NMII-B in MK polyploidization. |
