| Primary Identifier | MGI:4831137 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Cend1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The entire coding region and 1 kb of the 3' UTR were replaced with a lacZ gene and a floxed PGK-neo cassette via homologous recombination. Cre mediated recombination removed the neo cassette. RT-PCR, in situ hybridization and western blot analysis confirmed the absence of RNA and protein expression in homozygous mice. Beta-galactosidase expression is detected in neurons in the cortex, dentate gyrus, striatum and cerebellum where the endogenous protein is highly expressed. Expression of beta-galactosidase is targeted to the nucleus. |
